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Master training: Nanoparticulate X-Ray Computed Tomography Contrast Agents

Description

X-ray computed tomography (CT) has been widely used as a powerful diagnostic tool in clinics because is cost-effective, fast, and produces remarkable high-resolution 3D tomography of the anatomic structure based on the distinctive X-ray absorptions between different tissues.
Currently, CT contrast agents are mainly small iodinated molecules, which suffer from drawbacks such as short blood retention time, nonspecific in vivo biodistribution, and renal toxicity. Utilization of inorganic nanoparticles as potential X-ray CT contrast agents such as gold or hafnium oxide nanoparticles  (NPs) have gained recent attention due a high x-ray attenuation and non toxicity.1 Controlling structural characteristics (composition, mass concentration, size, shape and surface functionalization) are key parameters for optimized properties and functional performance. The surface coating of NPs determines also many of their physical and chemical properties, notably stability, solubility, and targeting.

The Bionanomaterial team of the LVTS, INSERM U1148 at University Paris 13 Bobigny, have a long experience on nanomaterials synthesis, with different composition, size, shape, structure and surface functionalization for bioassays, diagnosis and therapy. The inorganic core act both as a physical tool thanks to its specific magnetic2, plasmonic3 or luminescent4 properties and as a carrier to attach on its surface therapeutic molecules.  This latest was obtained by direct attachment at the surface or by grafting precursors groups for the covalent coupling (carbodiimine or click chemistry) of biological recognition molecules, such as antibodies or peptides.5 In particular, the team developed a non-aqueous sol-gel procedure to produce iron oxide NPs6  or near infrared persistent luminescence NPs4. A new direct synthesis of monodisperse water soluble bisphosphonate gold NPs were also just achieved3.

The team would like to extend the non-aqueous sol-gel procedure synthesis to hafnium oxide NPs and to explore the X-Ray contrast properties for gold and hafnium oxide NPs, depending on structural characteristic.

In the proposed research work, the objective will be to precisely control the gold and hafnium oxide NPs synthesis in term on size, shape and surface functionalization and evaluate “in vitro” and “in vivo” the potency of such X-Ray CT NPs. This work will be performed in close collaboration with the Pr. P. Ou, Inserm 1148, for X-ray CT imaging using FRIM platform in Bichat Hospital.

Skills : Knowledge in the fields of nanochemistry and nanomedecine are highly desirable. Good communication skills.

Caracterization techniques : UV-Visible; Infra-Red and fluorescence Spectroscopies, DLS, ThermoGravimetry Analysis, X-ray CT imaging

Keywords : nanoparticles, synthesis, characterization, nanomedecine

Contact : Laurence Motte Professeur   E-mail : Cette adresse e-mail est protégée contre les robots spammeurs. Vous devez activer le JavaScript pour la visualiser.

References : 1- T. L. McGinnity et al., Nanoscale, 2016,8, 13627-13637. 2-a) I. Milosevic et al. J. Phys. Chem. C 2011, 115, 18999-19004; b) C. de Montferrand et al. Small 2012, 8, 1945-1956; c) C. Goncalves et al. J. Mater. Chem. B 2013, 1, 5853-5864; d) Y. Lalatonne et al. Chem. Comm. 2008, 2553-2555. 3- a) R. Aufaure et al. RSC Advances 2014, 4, 59315-59322. b) R. Aufaure et al.Chem. Eur. J. 2016 DOI: 10.1002/chem.201602899
4-E. Teston et al. Chemistry – A European Journal 2015, 21, 7350–7354.   5-a) Bolley et al.Nanoscale 2013, 5, 11478-11489; b) S. Richard et al. J. Mater. Chem. B 2015, 3, 2939-2942; c) L. Motte et al.Faraday Disc. 2011, 149, 211-225; d) J. Bolley et al. Langmuir 2013, 29, 14639-14647. 6- S. Richard et al. Nanomedecine 10.2217/nnm-2016-0177